Coding

Part:BBa_K2603000:Design

Designed by: Cibele Zolnier Sousa do Nascimento, Fernando Satoshi Tutihashi   Group: iGEM18_USP-EEL-Brazil   (2018-10-09)


Lac PL - Laccase from Pleurotus ostreatus with RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 803
    Illegal BamHI site found at 486
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 994


Design Notes

Restriction sites for SpeI, XbaI, EcoRI and PstI were removed from the gene's sequence. The RBS sequence was taken from BBa_B0030. Biobrick prefix and suffix were add on the final part's sequence.

Primer Foward: TATATAGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGGCAG

Primer Reverse: TATATACTGCAGGCGGCCGCTACTAGTATTACCACTCAGC

Source

Sequence from Pleurotus ostreatus NRRL0366 laccase gene taken from NCBI and RBS sequence from iGEM's Registry.

References